All through the course of the LEAPS sequence of practicals you’ve got been gathering proof to check the next speculation: “Expression of proteins as a glutathione-S-transferase fusion permits fast and environment friendly purification utilizing affinity chromatography.” On this report you’ll be requested to offer some background details about the processes of making and expressing a fusion protein and the ideas of affinity chromatography. You’ll then be requested to current the info you collected and state how these knowledge contribute to the proof that helps (or refutes) the speculation. You’ll then be requested to offer a important appraisal of the experimental approaches we used and the info you collected.